Lauryl tryptose broth
Lauryl tryptose broth (LTB) is a selective growth medium (broth) for coliforms.[1]
Lauryl tryptose broth is used for the most probable number test of coliforms in waters, effluent or sewage. It acts as a confirmation test for lactose fermentation with gas production. Sodium lauryl sulfate inhibits organisms other than coliforms.
Formula in grams/litre (g/L)
- Tryptose: 20.0,
- Lactose : 5.0,
- Sodium chloride : 5.0,
- Dipotassium phosphate : 2.75,
- Potassium dihydrogen phosphate : 2.75,
- Sodium dodecyl sulfate : 0.1
- pH 6.8 ± 0.2
Samples positive for gas production are transferred to brilliant green lactose bile broth (BLGB) to detect the ability to grow in the presence of bile and produce gas at 95 °F (35 °C) for 48 hours. The absence of gas production in 48 hours is considered a negative test for coliforms.[2] Gas production serves as both a presumptive test and a confirmatory medium.
Fecal coliforms are distinguished from coliforms by growth in EC broth at 113.9 °F (45.5 °C) for 24 hours.
References
[edit]- ^ Mallmann, W. L.; Darby, C. W. (February 1941). "Uses of a Lauryl Sulfate Tryptose Broth for the Detection of Coliform Organisms". Am J Public Health Nations Health. 31 (2): 127–134. doi:10.2105/ajph.31.2.127. PMC 1531262. PMID 18015383.
- ^ Anne Maczulak (2011), "most probable number", Encyclopedia of Microbiology, pp. 542–545
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